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An engineered move 3 arrest mutant of DNA ligase would, if extensively Lively, pepper the genome with unresected 5′-adenylates at sites of DNA repair or joining of Okazaki fragments. This would be catastrophic if the extent of DNA 5′-adenylylation exceeds the mobile's capability to eliminate the lesions (33). This type of scenario is analogous to “poisoning” of topoisomerase IB by camptothecin prescription drugs or drug-mimetic enzyme mutations that selectively impede the DNA ligation phase from the topoisomerase pathway.
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